Generating a Proteomic Profile of Neurogenesis
Shaun Garnett is in the last stages of his PhD at the University of Cape Town. His thesis is focused on the proteomics analysis of human foetal neural stem cells. During his PhD he was fortunate enough to spend some time working the lab of Austin Smith at the Wellcome Trust Centre for Stem Cell Research in Cambridge (UK), learning neural stem cell culture. He also attended the Cold Spring Harbor Laboratories Proteomics Workshop, is an expert on all things Mass Spectrometry and an avid Bioinformatician.
In his talk he will discuss his current work focused on teasing apart the proteomic differences between human foetal hind-brain neuroepithelial stem cells and radial glia cultured in vitro. These cells proliferate in identical culture conditions but have vastly different morphology, proliferation rates and differentiation potential. Neuroepithelial cells have an epithelial morphology, rapid proliferation rate and form primarily neurons upon differentiation, while radial glia extend processes and are able to form all three neural tissue types.
Unravelling the molecular mechanisms of HIV associated neurocognitive disorders through mass spectrometry-based proteomics
Kim Gurwitz is a Training and Outreach Officer at the University of Cape Town (UCT) branch of H3 Africa Bioinformatics Network (H3ABioNet) – a pan African network of 32 bioinformatics institutions across 15 African countries. Her passion for knowledge transfer and youth training really comes to the fore in this role, in which she is coordinating an online introduction to bioinformatics course for students across the H3ABioNet network (among other exciting science communication projects).
Kim graduated from UCT with a BSc (with distinction) in Biochemistry and Genetics in 2012 and with a first class BSc (Medical) Honours degree in Medical Biochemistry in 2013. Her MSc in Medical Biochemistry thesis (2016) was an analysis of how HIV affects the brain using mass spectrometry-based proteomics techniques under the supervision of Professor Jonathan Blackburn (UCT).
Image of a neuroepithelial stem cell courtesy of Shaun Garnett